I need to express heterologous proteins in Bacillus (e.g. subtilis).
My first attempt, with an E. coli/gram-positive shuttle vector with Km
resistance, failed. I couldn't even get any transformants. I tried
another (Cm-resistance) plasmid and got transformants, but that plasmid
had already been cut and pasted beyond usefulness, so I couldn't do much
with it.
Does anyone have a suggestion on what vector you've used in Bacillus that
enabled you to (inducibly, even) express heterologous proteins and have
decent stability? The copy number does not have to be high. I also don't
want the proteins to be secreted.
Thank you for any and all suggestions!
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Frank Skraly
University of Wisconsin-Madison
skralyf at cae.wisc.edu
