Bacillus subtilis culture - a warning

John W. Patching 0001705s at Bodkin.UCG.IE
Thu Nov 23 10:10:18 EST 1995

Just a small word of warning. Be VERY careful with the handling and
disposal of Bacillus subtilis cultures, especially those that are
sporulating.  Once B. subtilis gets loose in a lab its very difficult to
get rid of and will give you major problems with contamination of plates,
fermenters etc. We used to grow B.subtilis in chemostats.  Inevitably some
got loose. It took us two days of  thorough disinfection (wash all surfaces
with bleach or alcohol etc.) before the problem was solved.

John W. Patching
The Martin Ryan Marine Science Institute
University College Galway, Ireland

Email: John.Patching at UCG.IE
Phone: +353-91-524411 Ext 2398
Fax:       +353-91-750456 or +353-91-525005

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