Derek & Nikki ALBERT wrote:
>> Could anyone tell me the best way to culture and then store this
> bacteria in any numbers
>> Regards Derek
1. Get your pure culture and inoculate a 100ml flask of an enriched
broth media. Incubate 24hr at 37dC on a shaker so you get a good,
turbid solution.
2. To eight flasks with 250mL each of a "sporulation broth" which I
think has low carbohydrates, inoculate with 10mL of the culture.
3. Shake n bake at R.T. for a couple of weeks. There will be a film
on the top. Gram stain should show 90%+ sporulation.
4. Spin down the stuff to get a nice thick tan paste.
5. Bring up to about 50mL with sterile saline.
6. Heat shock at about 60dC for a half hour or so. Store in the
fridge.
This will give a culture containing about 8 logs per 0.1mL and
should last at least a couple of years.
