How to circumvent DNA restriction in a bacterial host?

Cliff Mintz mintz at CNJ.DIGEX.NET
Tue Nov 21 11:21:26 EST 1995

	Dear Jim:

	From my experience, you have two options. You could try 
mating your construct into your recipient via conjugation from an r-m- 
strain of E. coli. This would tell you whether you have a restriction 
problem or a transformation problem. A second approach entails 
isolating a restriction minus strain of your recipient. We did this with 
Legionella and it is relatively easy. If you would like details, please 
contact me at mintz at cnj.digex.com.

	I hope this helps.

       Cliff Mintz, Ph.D			Phone:(908)940-6928
       Transcell Technologies Inc               Fax:(908)821-4470
       2000 Cornwall Road			e-mail:mintz at cnj.digex.com
       Monmouth Junction, NJ 08852
           	"No sacrifice is too great for science"-C.Adair 

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