Dear Jim:
From my experience, you have two options. You could try
mating your construct into your recipient via conjugation from an r-m-
strain of E. coli. This would tell you whether you have a restriction
problem or a transformation problem. A second approach entails
isolating a restriction minus strain of your recipient. We did this with
Legionella and it is relatively easy. If you would like details, please
contact me at mintz at cnj.digex.com.
I hope this helps.
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Cliff Mintz, Ph.D Phone:(908)940-6928
Transcell Technologies Inc Fax:(908)821-4470
2000 Cornwall Road e-mail:mintz at cnj.digex.com
Monmouth Junction, NJ 08852
"No sacrifice is too great for science"-C.Adair
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