Hi,
I am trying to do gene replacement in a gm(-) bacteria that contains an
uncharacterized restriction/modification system(s), however, I am not able to
get recombinant transformants via electroporation or CaCL2 methods. I assume
the problem is DNA restriction since I had a tough time cloning DNA from this
organism. I tried using several different R/M E. coli strains for preperation
of DNA without success. Does anyone have any suggestions or ideas on how I can
get arround this problem?
Thanks Jim.
