How to circumvent DNA restriction in a bacterial host?

Jim bina at unixg.ubc.ca
Tue Nov 21 05:33:37 EST 1995


I am trying to do gene replacement in a gm(-) bacteria that contains an 
uncharacterized restriction/modification system(s), however, I am not able to 
get recombinant transformants via electroporation or CaCL2 methods.  I assume 
the problem is DNA restriction since I had a tough time cloning DNA from this 
organism.  I tried using several different R/M E. coli strains for preperation 
of DNA without success.  Does anyone have any suggestions or ideas on how I can 
get arround this problem?

Thanks Jim.

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