Phosphate Precipitation in Casein solution

ron tate rtate at bmb-fs1.biochem.okstate.edu
Tue Nov 7 17:13:32 EST 1995

I need some help,  I use a vitamin defined media to grow lipoic acid 
deficient bacteria.  KPO4 is the buffer and charcoal treated casein is a 
major component.  I have made this media many times with no problems 
until recently.  Now I get what I think is CaPO4 precipitating when the 
pH is adjusted.  All reagents I have used before.  It's got me baffled, 
any suggestions.

Thanks in advance
ron tate 

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