In article <47dbf3$2is at news.duke.edu>, "Dr. David Rosen"
<drosen> wrote:
> Most forms of radiation cause mutations, usually by deleting
> a gene. UV light can induce mutations.
But, if I want to produce mutants of a specific species of a
filamentous fungus which I cannot get to sporulate, how would
you suggest I go about irradiating it?
> Some forms of nuclear radiation may be safe enough for a
> small laboratory (like an alpha or beta source),
Is there any advantage to using a radioactive source of
radiation instead of simply UV light?
> I don't know whether this is safe for a small laboratory.
> The chemical choline can induce chromosome anomolies, like
> chromosome doubling. However, it is a strong terotogen.
Choline? Isn't it just a nutrient? Are you sure it is toxic?
> Ozone can induce mutations: place the plants in an ozone
> filled containors.
Could you suggest a book or two on the subject? I.e., more on
the specifics of the actual procedures?
Thanks for your help!
--
Isak Lytting
ilytting at datashopper.dk
2:238/197.13
PGP fingerprint: FB 05 FB A6 34 F7 26 00 2F 81 AE EA B5 72 B3 15
Eschaton: 2012 Anno Domini
