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>Does anyone know if it is possible to create TBE buffer for electrophoresis? >If so, could you post or e-mail me the method? 5 x TBE From Sambrook et al.: per litre - 54 g Tris, 27.5 g boric acid, 20 mL 0.5M EDTA (pH 8.0) - for acrylamide electrophoresis use 1 x TBE - for agarose use 0.5 x TBE Share & Enjoy! Brent Pollock