Neither the two queries, nor the first reply are fair to the
present state of microbiology !!
1. Much effort has been made (successfully !) to bring order
to the pseudomonads.
2. Since long the genus Pseudomonas is restricted to de Ley
Superfamily II organisms, excluding Xanthomonas
(Stenotrophomonas), Comamonas, Acidovorax, Burkholderia,
Brevundimonas, etc. etc. - however it still merits further
taxonomic investigation using 16S rRNA sequencing in a
polyphasic approach.
3. If you want a name on your organism no. 2 - send it to a
specialist laboratory.
4. Are you sure organism no. 1 is Pseudomonas putida ?
5. If no. 2 is Pseudomonas putida, it could still belong to
a different biovar than no. 1 (You have to make a carefull
selection of the tests to use)
Our experience of the Pseudomonas fluorescens-complex is
that there is great variation in the biochemical test
patterns. We recommend 50 to 100 tests and a large database.
If biology was simple, it would not be fun !
E. F.
______________________________ Reply Separator _________________________________
Subject: Re: Pseudomonas identification
Author: SE {J.D.Brinck at bham.ac.uk}
Date: 1995-05-27 16.08
In article <hydex004.801522762 at gold.tc.umn.edu> hydex004 at gold.tc.umn.edu
(Frederick W Hyde) writes:
>From: hydex004 at gold.tc.umn.edu (Frederick W Hyde)
>Subject: Re: Pseudomonas identification
>Date: 26 May 1995 16:15:28 -0500
>Depends on species.
>Some species exhibit a characteristic pigment, usually fluorescein or
>pyocyanin, (green/blue, repsectively), when cultured on some solid media.
>the genus really is a catchall in some cases for organisms that don't
>have a particular home, and is only now being sorted out by DNA homology,
>fatty acid GC, and other molecular methods.
Ok, I'll narrow it down to a more precise question. I've got 2 bugs, 1's a
Pseudonomas putida, the other one is unknown. Both are Gram -ve and oxidase
+ve. What other tests can I do which could show a difference between the 2?
Thanks for the input,
Jason Brinck,
School of Biochemistry,
University of Birmingham
************ CCUG = Bacterial strains & Identification *************
Enevold Falsen, Civ.ing. - Curator Voice: +46.31.60 46 25
Culture Collection, University of Goteborg Fax: +46.31.82 54 84
Guldhedsg 10, S-413 46 Goteborg, Sweden IN: <ccugef at ccug.gu.se>
********************************************************************
