In article <3qbi04$akk at mserv1.dl.ac.uk> <Enevold.Falsen at alinks.se> writes:
>From: <Enevold.Falsen at alinks.se>
>Subject: Re[2]: Pseudomonas identification
>Date: 29 May 1995 05:19:16 +0100
>Neither the two queries, nor the first reply are fair to the
>present state of microbiology !!
<Points 1. and 2. not related to my original Q>
>3. If you want a name on your organism no. 2 - send it to a
>specialist laboratory.
Answer: No, I just want to be sure its not a P.putida
>4. Are you sure organism no. 1 is Pseudomonas putida ?
Answer: Yes. - Or else its 21/2 years of my PhD down the drain :-)
>5. If no. 2 is Pseudomonas putida, it could still belong to
>a different biovar than no. 1 (You have to make a carefull
>selection of the tests to use)
Answer: Amy Lotto suggested doing tests for denitrification, gelatin
hydrolysis and pigmentation. The results from these tests should be the
same for both biovar A and B according to my Bergey's Manual of Determinative
Bacteriology
>Our experience of the Pseudomonas fluorescens-complex is
>that there is great variation in the biochemical test
>patterns. We recommend 50 to 100 tests and a large database.
I'm afraid I haven't got time, this is just a minor sideline to my project!
Anyway, thanks for the input,
Jason Brinck
School of Biochemistry,
Birmingham University
>If biology was simple, it would not be fun !
At the moment, boredom with results would suit me fine :-}
>E. F.
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