In article <3prlmdINNhvh at woozle.mel.dbe.CSIRO.AU> andreww <andreww> writes:
>I would say a big NO to measuring Strepto's by OD. The filamentous nature of
>the beast makes even a representative sample near impossible.
>When I used to grow them I found measuring a cell pellet volume and measuring
>the dry weight was the best way to follow thier growth.
>good luck tho,
>Andrew Wolfe, csiro DBE, Parkville, Vic.
>
We found some years ago (my then student Gerard Markx did this particular expt)
that the dielectric method works perfectly well for Streptomyces spp. Ralf
Fehrenback, Martin Comberback et al published nice traces with Streptomyces in
Fehrenbach, R., Comberbach, M. & Pjtre, J.O. (1992) On-line biomass monitoring by
capacitance measurement. J. Biotechnol. 23, 303-314.
We also published several papers on solid state fermentations of the filamentous
Rhizopus oligosporus (making tempe) usaing the dielectric method:
Davey, C.L., Penaloza, W., Kell, D.B. & Hedger, J.N. (1991) Real- time monitoring of
the accretion of Rhizopus oligosporus biomass during the solid-substrate tempe
fermentation. World J. Microbiol. Biotechnol. 7, 248-259.
Penaloza, W., Davey, C.L., Hedger, J.N. & Kell, D.B. (1991) Stimulation by potassium of
the growth of Rhizopus oligosporus during liquid- and solid-substrate fermentations.
World J. Microbiol. Biotechnol. 7, 260-268.
Penaloza, W., Davey, C.L., Hedger, J.N. & Kell, D.B. (1992) Physiological studies on the
quinoa tempe fermentation, using on-line measurements of fungal biomass production. J.
Sci. Food Agric. 59, 227-235.
Fuller details on the web at URL http://144.124.112.51/dbk/ai/dielec.htm
or via our home page on http://144.124.112.51/home.htm
Douglas.
