IUBio

Streptomyces growth measurement

Leonid V Bystrykh Bystrykh at biol.rug.nl
Thu May 25 10:32:58 EST 1995


andreww <andreww> wrote:
>
> I would say a big NO to measuring Strepto's by OD. The filamentous nature of 
> the beast makes even a representative sample near impossible.
> When I used to grow them I found measuring a cell pellet volume and measuring
> the dry weight was the best way to follow thier growth.
> good luck tho,
> Andrew Wolfe, csiro DBE, Parkville, Vic.
> 
Actually, I also doubted about validity of OD assay in case of S.coelicolor A3(2).
However, when it was compared with dry weight of the same cells I got
nice straight line with error of less than 10% at least.Thus, i would not say big NO.

Lenja



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