Dear Micronetters:
I am trying to break open S. aureus to isolate peptidoglycan
precursors. In the literature, people usually grind staphylococci with
glass beads in an omnimixer. Is there a way to get good breakage with a
sonicator? I do not have a French Press in the lab. and I cannot use
lysozyme. Any thoughts or suggestions would be welcome. Alternatively,
if someone out there would like to part with an Omnimixer, please let me
know.
TIA
******************************************************************************
Cliff Mintz, Ph.D Phone:(908)940-6928
Transcell Technologies Inc Fax:(908)821-4470
2000 Cornwall Road e-mail:mintz at cnj.digex.com
Monmouth Junction, NJ 08852
"No sacrifice is too great for science"-C.Adair
******************************************************************************
