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Hyphomicrobium ZV 580 growth problems

unb11a at ibm.rhrz.uni-bonn.de unb11a at ibm.rhrz.uni-bonn.de
Thu May 18 14:09:54 EST 1995


Hi everybody!
I´m in big trouble with my Hyphomicrobium zavarzinii strain ZV 580! In liquid 
culture (bottles of 15 litres), they stop growing after reaching an optical 
density of only 0.2-0.3 without any apparent reason. In earlier times, the 
grow up to an OD of 2.2. There are no contaminations observed. I need an 
amount of 100 grams of cells for each preparation of a dye-linked formaldehyde 
dehydrogenase. Is anybody out there who works with this strain, too?

Thanks in advance,
Marcus


Marcus O. Lueg
Institute of Botany
University of Bonn
Germany
E-Mail: unb11a at ibm.rhrz.uni-bonn.de



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