IUBio

help on staining

Ino Sofjan inosof at acs.tamu.edu
Wed May 17 13:25:54 EST 1995


Can anybody out there help me with staining tricks?  We've been trying
to enumerate respiring and non-respiring bacteria (pseudomonas
and flavobacteria) using fluorescence microscopy, but so far results
are questionable at best.  We are currently using a combination of
Acridine Orange and INT dyes.

Any suggestions and ideas?  Different stains or other procedures are
most welcome.


TIA


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