Hi Folks,
I want to select for GalE in a normal delta(lac-proAB) host. Any
ideas how? I have a GalE lacZ host for transduction from but there is
no transposon to help in the selection.
Why do I want a GalE delta(lac-proAB) host? If you use pUC18 in such
a host with IPTG/Xgal etc the alpha complementation is lethal with
phenylgalactoside. This gives a direct selection for whites. Just
think, no phosphatasing and everything has an insert.
Many thanks
Duncan
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My mind's made up. Don't confuse me with the facts!
