In article <1995Mar5.205122.19197 at lafn.org> ak507 at lafn.org (Pong Nim) writes:
>From: ak507 at lafn.org (Pong Nim)
>Subject: Need help on growing Clostridium & Lactococcus
>Date: Sun, 5 Mar 1995 20:51:22 GMT
Most Lactobacilli (and Lactococcus) are relatively fastidious. Your
lactococcus should grow well in MRS media from Difco laboratories. It's a
dehydrated broth medium. To make plates, just add 15g/L agar before
autoclaving. 30C should work and it's not necessary to maintain strict
anaerobic conditions. The colonies will likely be pinpoint, raised,
smooth, round, butyrous, off-white. Transfer every 2-3 days if you're not
going to maintain a frozen culture. Alternately, if you have a variety of
common chemicals at your disposal, you can make a medium described by
Friedman and Gaden (Biotech. Bioeng. 12, 961-974, 1970). It contains
CaCO3. The plates are cloudy and when the organism produces acid, the
plate clarifies. Your students might enjoy that.
>HI THERE:
>I am trying to grow Clostridium sporogenes & Lactococcus lactis
>the Lactococcus
>nder what condition to maintain the culture. Thank you kindly.
>--
>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
>Pong Nim
>LAMC
