Growth of Propionibacterium acnes
If you want to isolate (obtain high colony counts) of
Propionibacterium acnes on human skin, you should use 5% (horse)
blood in Columbia agar. You will than get many colonies, but small
colonies after 48h anaerobic incubation.
If by any chance you would like to have high yields (mass
production) of the organism, you should use liquid medium,
start with high inoculum and increase oxygen concentration
slowly. P.acnes is VERY oxygen tolerant.
E. Falsen
************ CCUG = Bacterial strains & Identification *************
Enevold Falsen, Civ.ing. - Curator Voice: +46.31.60 46 25
Culture Collection, University of Goteborg Fax: +46.31.82 54 84
Guldhedsg 10, S-413 46 Goteborg, Sweden IN: <ccugef at ccug.gu.se>
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