Got some questions on a DNA subclone/restriction map lab I did.
1. In one well of my gel separation, I saw a bright haze in the
bottom lane rather than a band. Is the sample degrading as a goes
across the gel? What’s going on? (Used ethidium bromide with E.
coli vector)
2. Why ultracentrifuge to purify DNA?
3. Why would a restriction enzyme NOT cut a plasmid? Because a
protein in gel bound to restriction site? Why partial digests?
Thanks in advance, Dave
