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SKNMC Cell Line HELP?

Lois C. Rich Rich_Lois_C.PRILVMS1 at msmail.bms.com
Thu Jun 29 06:32:26 EST 1995


I am presently working with these cells in tissue culture- from the ATCC. 
They are giving m problems growing and splitting.  I am growing them in
Eagle's MEM + Earles, _ nonessential amino acids + pyruvate + 10% FCS. 
They slough when they are near confluence.  Any ideas out there?
TIA

Lois C. Rich
Dept. of Metabolic Diseases
Bristol-Myers Squibb
Princeton. NJ



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