I am presently working with these cells in tissue culture- from the ATCC.
They are giving m problems growing and splitting. I am growing them in
Eagle's MEM + Earles, _ nonessential amino acids + pyruvate + 10% FCS.
They slough when they are near confluence. Any ideas out there?
TIA
Lois C. Rich
Dept. of Metabolic Diseases
Bristol-Myers Squibb
Princeton. NJ