benedik at uh.edu (benedik at uh.edu) writes:
> I was reading in the Gibco/BRL catalog that strains carrying the deoR
> mutation allows improved uptake of large plasmids.
>> Does anyone know the basis for this? Is it improved transformation by
> large plasmids? Improved replication? Would this have an impact on
> production of large amounts of plasmid DNA even from smaller plasmids?
>>deoR is (as i recall) is a negative regulator for the synthesis
of deoxyribonucleotides. By knocking it out the cell overproduces
dNTPs which favors the continued replication of plasmids,
paticularly if the are large..