I'am gone use Pseudomonas putida in exogenous plasmid isolation. I want to use the Eckhardt
procedure fore screening, hoping to catch the big plasmids. Unfortunately this Pseudomonas strain
seems very hard to get lysed with lysozyme, RNase and protease treatment. So if there is anyone
out there who had tried Pseudomonas strains with the Eckhardt technique, please shear your
experience with me. Also: If there is another successful method for screening Pseudomonas in big
scale, I would appreciate hearing from you.
Thank you,
Karine Dronen
e-mail address: Karine.Dronen at im.uib.no
