Hello,
I am attempting to grow Thermotoga maritima in small scale culture. So
far the MMS media described by the culture collection (DSM) has been
horrible! It contains loads of Na2S and precipitates like crazy, is other
media effective?
Any experience with Difco Marine Broth or SSM media? Also, how are long
term stocks (-70°C) made? I tried glycerol and managed to lyse the usually
tough little bugger... How is growth at lower pressures than the described
300kPa?
Help!
Feel free to Email any insight, it is much appreciated
Ted Michelini
University of Oregon
Institute of Molecular Biology
tedm at darkwing.uoregon.edu
