IUBio

help! - EMS mutagenesis

JLeachCO jleachco at aol.com
Mon Jul 17 10:14:57 EST 1995


The key to knowing if you are in the right concentration ranges and time
of treatment is to check "% kill" (i.e. % of the population that doesn't
survive the treatment) and look for a marker which is easy to find (such
as auxotrophy or a drug resistance) to ensure you are really mutating.

Each bug may require some juggling to find the optimum mutation
conditions.   



More information about the Microbio mailing list

Send comments to us at biosci-help [At] net.bio.net