I am working on the purification of monoclonal mouse antibody IgG2b for
my thesis work. I am trying to use hollow fiber modules modified with protein A
or protein G. I am also planning on immobilizing anti-mouse IgG, in the near
future. So far I have been unsuccessful in getting high capacities with protein
A or protein G.
I would appreciate if anyone of you could highlight me on any special
conditions/buffers that I might have to use in order to get high capacities.
Please reply to the following e-mail address:
sjwaig01 at ulkyvm.louisville.edu
Thanks in advance.