From: Potter Wickware <wick at netcom.com>
Subject: Re: purifying Thiobacillus/Cyanobacteria
To: P.Cassidy at aqua.utas.edu.au
cc: microbiology at net.bio.net
In-Reply-To: <P.Cassidy-120795134005 at mpg7-97.appbio.utas.edu.au>
Message-ID: <Pine.3.89.9507120953.A19073-0100000 at netcom11>
Content-Type: TEXT/PLAIN; charset=US-ASCII
Why don't you first amplify the T ferro by inoculating into iron broth,
pH <3. That part is easy. Then plate: may have to use agarose or silica
gel for plate matrix, or streak on filter papers and then float on iron
broth. Tedious, but usually works, eventually.
As for the halophiles, I dunno. Never tried it. I never heard of
halpohiles and acidophiles coexisting. Where's this site?
On 12 Jul 1995 P.Cassidy at aqua.utas.edu.au wrote:
> I am currently, trying my hardest, to purify enviromental
> samples of halophilic Cyanobacteria, and samples of
> Thiobacillus ferrooxidans from a stream contaminated with
> acid mine drainage.
>> Problem being, nothing quite works the way others have reported.
>> So I was wondering if anybody here purfied any of these
> and could give me some guidance 'cause I need help