IUBio Biosequences .. Software .. Molbio soft .. Network News .. FTP

QUESTION: Borrellia testing on csf

Bryan Kiehl b3748 at cts.com
Sat Jul 8 11:40:40 EST 1995

In article <3ti593$sg0 at lastactionhero.rs.itd.umich.edu>,
   jperrin at umich.edu (John Perrin) wrote:
>I am posting this question for anyone that might do testing for Borrellia 
>antibodies in spinal fluid.  1.) How do you process csf for western 
>blot?  2.) Are your samples diluted for testing as serum specimens are or 
>are they run without dilutions?  3.)  What types of interpretations do 
>you use? i.e. If you obtain an equivicol result on a non diluted specimen 
>is that clinically significant?
>Please respond to either this posting or e-mail any answers you might 
>have directly to me at  jperrin at umich.edu
>                                        Thanks in advance.
There has been a variety of methods used and none are well accepted. One 
approach is to measure Ig concentrations in serum and CSF and adjust values 
based on a ratio. Different ratios have been used. Others simply test 
undiluted or moderately diluted CSF. Since the concentrations of the various 
proteins in the western are not well standardized and there is some variation 
of band locations seen across the blot, the CSF concentrations used are 
probably not that important.

I work in a commercial diagnostic company that does not make westerns, 
although we use the technique for development. If I were to design a 
commercial method, we would need to show respectable specificity (>95%) first 
and then test sensitivity. For borrelia, you also need to test CSF from 
patients with symptoms similar to borreliosis and documented not to have 
borreliosis. Using samples from asymptomatic subjects don't really predict 
specificity. Typically the FDA requires 50-200 such samples be tested. You 
would probably test at several CSF dilutions or you could vary borrelia 
antigen concentrations in the blot. After you have satisfactory specificity, 
then you can see how sensitive the test is. That's assuming you have enough 
defined samples to estimate sensitivity. Obviously, you also must define the 
interpretation criteria used in the western and keep it consistent.

You may also wish to include known false positive type sera. For borrelia, the 
 best recognized "problem sera" usually affect IgM blots. These are EBV, 
parvovirus, and rheumatological serology positives. In addition, other 
spirochetal infections (syphilis, relapsing fever, etc) cause significant 
cross-reactivity. We have also observed that H. pylori serology positive 
samples signficantly cross-react with flagellin.

One reference I might suggest are a series of articles by Paul Fawcett at the 
DuPont Institute or a recent article in J. Clin. Micro by Russ Johnson from 
U. of Minnesota. They has addressed many of the above issues for western 
blots, but don't say much about CSF. Since neuroborreliosis is more 
common in Europe, there are also several articles from Europe discussing the 
CSF issue. However, none offer a clear-cut method that can be applied without 

Bryan Kiehl
15222-A Avenue of Science
San Diego, CA 92128
(619) 592-9300, ext 309
(619) 592-9400 [FAX]

More information about the Microbio mailing list

Send comments to us at biosci-help [At] net.bio.net