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CURRICULUM VITAE
Family name: GAUTHIER
First name: Martine
Date of birth: 1969/10/28
Nationality: Canadian
Adress: 2351, boulevard Père Lelièvre
apt. 305
Québec, (Québec) G1P 2X2
Telephone: (418) 682-5758
Fax: (418) 656-2861 (Université Laval)
ACADEMIC QUALIFICATIONS
YEAR INSTITUTION
DEGREE OBTAINED
1995 Université Laval
M. Sc. in microbiology
Sainte-Foy (Québec)
1993 Université Laval
B. Sc. in microbiology
Sainte-Foy (Québec)
PROFESSIONNNAL EXPERIENCE
September 1993-june 1995
Subject of MSc. thesis : Phenotypic characterization of the
(ptsH) of Streptococcus salivarius G22.4: function of P-Ser-HPr
in the regulation of sugar utilization in S. salivarius.
Director: Dr. Christian Vadeboncoeur
Objectives:
(1) Compare the wild-type S. salivarius ATCC 25975 strain to the
G22.4 mutant.
(2) Determine the generation times when these two strains are
grown on glucose, fructose, mannose, galactose and melibiose.
(3) Study the phosphorylation of the HPr protein (product of the
pts gene) on serine-46 using [32y-P]-ATP, and study the
phosphorylation of the Elll protein using [32-P]-PEP.
(4) Study diauxic growth patterns in liquid media containing two
sugars (one PTS and the other non-PTS).
(5) Determine -galactosidase, B-galactosidase and galactokinase
activities in the presence of glucose, fructose and their
corresponding inducers.
(6) Determine the concentrations of the four forms of HPr
(HPs-Ser-P, HPr-His-P, HPr-Ser-His-P, free HPr) during the
exponential growth phase.
(7) Study the inducer exclusion in resting cells in the presence
of two sugars, one PTS (glucose or fructose) and one non-PTS
(lactose, galactose or melibiose).
Techniques used:
(1) Bacterial cultures.
(2) Separation of bacterial cell components by alumina grinding
and ultracentrifugation.
(3) Polyacrylamide gel electrophoresis and autoradiography.
(4) Radioactive phosphorylation procedures using [32y-P]-ATP and
[32-P]-PEP.
(5) Measuring the concentrations of the sugars and the activities
of the enzymes mentioned above using common quantitative methods,
including 14C for measuring galactokinase actvity.
(6) Quantifying the phosphorylated forms of HPr by crossed
immunoelectrophoresis.
May - August 1993
May - August 1992
Research training in the Department of Infectology of the Centre
Hospitalier de l'Université Laval, Sainte-Foy, Québec.
Objectives:
(1) Optimize the growth of Toxoplasma gondii in human macrophages
(U937) and in bovine fibroblasts (BT) in order to establish an
in vitro system for further studies.
(2) Determine the inhibition potential against T. gondii of
several purine analogs (5 allopurinol derivatives and 2 formycin
derivatives).
(3) Study the incorporation of radioactive elements into the
nucleic acids and proteins of T. gondii in the presence of the
analogs using the two in vitro systems.
Techniques used:
(1) Cell cultures (growth T. gondii using human and mouse
macrophage and bovine fibroblasts).
(2) Evaluation of the growth or growth inhibition of T. gondii in
the presence of analogs using radioactive markers (3H, 35S) and a
staining technique developed in the laboratory where I worked.
(3) Phase-contrast and optical microscopy for counting cells.
(4) Ultraviolet spectroscopy.
OTHER EXPERIENCE
January - April 1995
Teaching assistant
Biochemistry laboratoty, Department of Biochemistry, Université
Laval, Sainte-Foy, Québec, Canada.
June - August 1991
Assistant biologist
Ministère du Loisir, de la Chasse et de la Pêche, Service de
l'Exploitation de la Faune, Hull, Québec, Canada.
PUBLICATIONS
Maion, G., M. Gauthier and S. Chamberland, 1993. Purine Analogs
as Potential Antiparasitic Agents against Toxoplasma gondii.
American Society for Microbiology Annual Meeting, Atlanta,
Georgia, USA.
Chamberland, S., G. Maion, L. Cantin and M. Gauthier, 1993.
Evaluation of the activity of classic purine analogs against
Toxoplasma gondii. Annual Meeting of the Canadian Society for
Clinical Investigation, Vancouver, B.C., Canada.
Maion, G., L. Cantin, M. Gauthier and S. Chamberland, 1995.
Purine Analogs as Potential Antiparasitic Agents against
Toxoplasma gondii. Journal of Infectious Diseases, (submitted).
References will be provided on request.
