In article <3g66hv$a6v at mserv1.dl.ac.uk> mbpln at s-crim1.dl.ac.uk (M.J. Pallen) writes:
Does anyone have a handy way of scoring the
>relA-negative phenotype, so that I can check that I really do have a relA
>mutation in the second strain? Cahel & Rudd in Neidhardt's E. coli & S.
>typhimurium book mention SMG medium. Is this the best way of
>distinguishing stringent from relaxed strains?
The method I used was serine sensitivity. For a useful reference, see Uzan &
Danchin, Biochem. Biophys. Res. Commun. (1976), vol. 69, p. 751-758. "A rapid
test for the relA mutation in Escherichia coli".
all the best,
Michael Kertesz
ETH-Microbiology,
Zurich, Switzerland.
