------- Forwarded Message Follows -------
Date: Tue, 24 Jan 1995 09:49:02 GMT
From: jshtsang at hkucc.hku.hk
Reply-to: jshtsang at hkucc.hku.hk
To: "bionet.microbiology mail newsgroup" <bionet-news at dl.ac.uk>
Subject: Re: Non-denaturing SDS-PAGE
>I think you can try to use glass beads (size of 40mesh or smaller). Just
>put the sterilised (if necessary) glassbeads into the cell paste up to the
>miniscus and vortex the tube for 20 seconds, cool (to prevent excess heat
>formation) and repeat the vortex for a couple of times. Wash the glassbeads
>cell debris with buffer and spin the collected lysate for a clear lysate.
>Analyse the protein concentration and load sample on PAGE. Try this on non-
>labelled cells first so you know whether it works or not. I have use this
>method on E. coli before and it works.
- Wanted: a source for suitable glass beads
- Supplier name, phone number, fax, email, etc... would be greatly
appreciated (pllease, international suppliers only)
Thanks a lot !
GC
Giuseppe Cornaglia
Istituto di Microbiologia
Strada Le Grazie, 8
37134 Verona (Italy)
Tel. 045-8098196
Fax 045-584606
e-mail giuseppe at borgoroma.univr.it
