This subject has been up already ?
Ultrasound - X-press - Grinding - Shaking with beads - Chemical - etc.
For Brucella you could try disruption with alumina Al2O3.
You simply grind the wet cells with powder of Al2O3. Sigma used
to have a special grade. You can autoclave the mortel.
A low cost method we have used for 20 years.
E. Falsen, CCUG
