Hello! I'm going to run some 35SMet-labeled Brucella
(Gram-negative) whole cell proteins on non-denaturing
SDS-PAGE. In order to keep the proteins at non-denature state I can't use SDS and other detergent to lyse the
cells, I know I can sonicate the cells, but that would be my
last resort, because I don't want to contaminate our
sonicator with 35S. So is there any other method availabel
that can lyse the cells? Any other suggestions on non-
denaturing SDS-PAGE would be appreciated too. Thanks!
Guobin Song
Dept. Vet. Pathobiology
TAMU, TX
email: gsong at vetmed.tamu.edu
