At 01:32 PM 1/19/95, Sandra Young wrote:
>>Would someone be able to provide me with a simple to follow methodology for
>testing oxidation/fermentation, using Hugh Leifson media.
>Thank-you in advance
>Sandra
>>Sandra:
Inoculate 2 tubes of Hugh Leifson media. Overlay one with sterile mineral
oil (or strictly speaking you should use sterile petrolatum to insure good
anaerobic conditions). Incubate for 48 hrs or in some cases it may take up
to 14 days for reactions to occur. If both tubes turn acidic the organism is
considered a fermenter, if only the tube without oil turns acidic the
organism is considered an oxidizer and if no acidity is detected in either
tube, the organism is considered a nonutilizer of the sugar. Hope this helps.
Ben
