Does anybody know concentration of molybdenum that will inhibit PCR?
I've got a danmed feces specimen that contains 10 ppm molybdenum ... DNA
extracted from this specimen is inhibitory down to 1/100 dilution!
I've tried several sequential DNA extractions, alchohol ppt'ns ... on
1/2 of my DNA sample, I've also tried competing the Mo with Lithium
chloride ... no improvement ... likewise with exposure to EGTA and EDTA!
I'm considering chelex.
Any suggestions? Molbdenum can have +6 charge!
thanks a bunch,
Andre
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Andre Hamel email: hamel at cc.umanitoba.ca
Manitoba Government tel: 204/945-7630
Department of Agriculture FAX: 204/945-8062
Veterinary Services Branch
Winnipeg, Manitoba, CANADA
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