Based on my experience, rpsL does not confer spectinomycin
resistant to the strain that carry the mutation. However, spectinomycin is
difficult to work with on certain strains of E. coli. I have also
encountered low levels of Spr in these strains. Raising the
concentration as you suggest should do the trick.
Cliff Mintz
On Tue, 17 Jan 1995,
Duncan Clark wrote:
> Hi Folks,
>> Does anyone know if an rpsL E.coli ie streptomycin resistant is
> automatically spectinomycin resistant? I have the Str/Sp gene
> from Tn7 on a multicopy plasmid (one of the few antibiotic genes that
> is stable on v.high copy vectors - the others being Amp and Kan) and
> use spectinomycin 50ug/ml as my selection. Unfortunately I am finding
> that my normal strains of choice for other applications are rpsL and
> give a detectable background on spectinomycin plates. I will take the
> concn up to 200ug/ml which I know the plasmid quite happily copes
> with and see if that does the trick. However it would be nice to know
> if rpsL is automatically Sp resistant.
>> Thanks
>> Duncan
>> --
> -----------------------------------------------------------------------------
> My mind's made up. Don't confuse me with the facts!
>>>
