Hi Folks,
Does anyone know if an rpsL E.coli ie streptomycin resistant is
automatically spectinomycin resistant? I have the Str/Sp gene
from Tn7 on a multicopy plasmid (one of the few antibiotic genes that
is stable on v.high copy vectors - the others being Amp and Kan) and
use spectinomycin 50ug/ml as my selection. Unfortunately I am finding
that my normal strains of choice for other applications are rpsL and
give a detectable background on spectinomycin plates. I will take the
concn up to 200ug/ml which I know the plasmid quite happily copes
with and see if that does the trick. However it would be nice to know
if rpsL is automatically Sp resistant.
Thanks
Duncan
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My mind's made up. Don't confuse me with the facts!
