>>We are currently looking for an alternative to 'velvets' for replica-plating
>>of bacterial spread cultures. Does anyone currently use or know of a good
>>alternative or commercial apparatus.
>>Thanks for any replies in advance.
>I have been using Whatman 541 filters and a glass coffee jar. Put about 3
Kimwipes on top of the coffee jar and secure them with a rubber band. Place
541 filter on top of kimwipes (with orientation mark),using a forceps. Then
press spread plate down on filter. Hold filter again with forceps and
remove master spread plate. Put replica plates on top of the filter to
transfer colonies.
This method also has the advantage that after transfer of colonies, the
filter can be incubated on the surface of another plate and then used for a
dot blot.
Regards
Ronan O'Kennedy
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Ronan O'Kennedy
Growth and Fermenatation Technology Lab
Dept of Microbiology
University College Galway
Ireland
Phone ***-91-24411ext 2191
Fax ***-91-25700
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