In article <78932379934n12 at 134.58.40.4>, Chris=Michiels%LMM%AGR at agr.kuleuven.ac.be says:
>>I read in a microbiology textbook that synchronous growth can be the result of
>temperature changes, nutrient limitation and other manipulations.
>Could someone tell me how synchronization is generally achieved for E. coli,
>and how it can be easily monitored?
>Is it possible that just diluting an overnight E. coli broth culture grown at 37°C
>into fresh medium at 30°C is sufficient for a few synchronized divisions?
You can not synchronize E.coli by subculturing them.
One of the best ways is to set up a chemostat and adjust the doubling time
of the culture so that to adjust the growth rate. There are lots of
references out there about setting up chemostats.
Another way is to work with a temperature sensitive mutant (that is not
a lethal mutation). Raise the temprature to that point to stop growth
then shift the temperature down to normal (say 37). this would provise
synchrony for a while.
Good Luck
Feras
