I have used the Kado/Liu procedure to isolate plasmids of 100-135
kb. It is quick, simple and works well. I would try it and see what happens.
Good luck
Cliff Mintz
On Wed, 4 Jan 1995 Andrea=Marconi%Algemeen%IM.WAU at Vines.WAU.NL wrote:
>>> >If anyone has a surefire method to isolate large plasmids from E. coli
> >I would appreciate a post to the group or an Email. I am aware of the
> >usual Kado/liu but if there is any other suggestions I would appreciate it.
> >The plasmid I am going after is about 90kb.
> >THanks in advance
>> Maybe you just tried and didn't work but I used to work with E. coli strains transformed
> with 50-60 kb plasmids (from a gene bank) and I didn't do anything else than the normal
> miniprep (like is described by Maniatis ) without any problem. The DNA once extracted was
> used to transform again the same strain and was working.
>> Andrea
>>>>
