>If anyone has a surefire method to isolate large plasmids from E. coli
>I would appreciate a post to the group or an Email. I am aware of the
>usual Kado/liu but if there is any other suggestions I would appreciate it.
>The plasmid I am going after is about 90kb.
>THanks in advance
Maybe you just tried and didn't work but I used to work with E. coli strains transformed
with 50-60 kb plasmids (from a gene bank) and I didn't do anything else than the normal
miniprep (like is described by Maniatis ) without any problem. The DNA once extracted was
used to transform again the same strain and was working.
Andrea
