In article <3golo6$mbv at masala.cc.uh.edu> benedik at uh.edu (benedik at uh.edu) writes:
>We are doing some work with different isolates of Pseudomonads, some of
>which we know what they are and some we don't. I would like to put in
>place some simple diagnostic system to verify which strains we are
>working on. I don't really care that I find out precisely what they
>are, but I want to be able to unambiguously distinguish between a few
>strains on a repeatable basis. In other words we are doing some
>genetics and transferring plasmids between strains and I want to be
>sure that my putative strain from a cross is really a new exconjugant
>and not a contaminant or revertant. There are no useful phenotypes in
>hand (except on the plasmid).
>Are there some diagnostic strips or microtiter dish systems that I can
>utilize that have lots of different tests so that I most likely can
>distinguish between these strains.
>Suggestions or pointers would be quite welcome.
API do strips that consist of microtubes containing dehydrated substrates.
These tests are inoculated with a bacterial suspension which reconstitutes the
media. During incubation, metabolism produces colour changes that are either
spontaneous or revealed by the addition of reagents.
Two kits for Pseudomanas spring to mind the API 20 E (which has 20 tests) and
the ATB 32 GN (which has 32 tests). Both are marketed for the identification
of gram negative rods. The latter (ATB) is automated but can be read
manually and is reputed to identify 19 different Pseudomonas. As a
confirmatory tool to check that you are isolating your origin strain it should
work well.
Their address is:
API Sytem - La Balme Les Grottes 38390 Montalieu Vercieu France
Tel 74 90 66 77
Regards
Dr Roy Goodacre E-mail: rrg at aber.ac.uk
FTP: 144.124.112.36 Voice: +44 970 623111 ext 4187 (work)
Fax: +44 970 622354 +44 974 241236 (home)
URL: http://gepasi.dbs.aber.ac.uk/roy/chemom.htm
Snail: Inst Biol Sci, University of Wales, Aberystwyth, Dyfed, UK
