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id <01HMKFQDDAE8007CT3 at hal.hahnemann.edu>; Thu, 2 Feb 1995 08:25:39 EST
Date: Thu, 02 Feb 1995 08:25:38 -0500 (EST)
From: MCGEED at HAL.HAHNEMANN.EDU
Subject: Re: help on case study!!
In-reply-to: <amthomps.16.2F30233E at zebu.cvm.msu.edu>
To: AMY THOMPSON <amthomps at zebu.cvm.msu.edu>
Cc: microbiology at net.bio.net
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On Thu, 2 Feb 1995, AMY THOMPSON wrote:
> Hi. I am doing a case study and need some help with it. Here is the case:
> A neutered tomcat has been ill for several days and had become progressively
> worse, showing increasing cough, labored breathing, and failure to eat.
> Consequently, its owner brought it to the clinic. In addition to
> the foregoing symptoms of cough, dyspnea, and inappetence, clinical signs
> detected by the vet included an elevated temp. of 103.9 and a bilateral
> mucopurulent nasal discharge. Auscultation revealed medium crackles lung
> sounds. Laboratory test results included a CBC in which the differential
> count showed and elevated WBC count with a moderate left shift. The
> urinalysis was normal. A fecal examination was negative. A gram stain of
> lung aspirate revealed gram negative rods. These bacteria grew well as mucoid
> colonies on MacConkey agar. They were lactose-positive, oxidase-negative, and
> non-motile.
>> I have a few questions.
> 1. What is MacConkey agar? Does it matter?
> 2. What are the possible etiologic agents? Why?
> 3. What etiologic agents can be ruled out?
> 4. What precautions should be undertaken before doing antimicrobial therapy?
>> I would appreciate ANY help you can give me regarding this assignment. Send
> responses to amthomps at zebu.cvm.msu.edu>> Thank you!!
>>Sounds a lot like Klebsiella pneumoniae or a related species. MacConkey
[selective and differential media] only grows up Gram (-) bugs [selective]
and differentiates lactose fermenters from lactose nonfermenters
[differential]. The big three that are lactose fermenters are
Enterobacter, E. coli, and Klebsiella. Klebsiella forms characteristic
mucoid colonies on this media (due at least in part to the capsule it
makes), and is nonmotile. I'm not sure what it's oxidase reaction is,
but I think all Enterbacteriaceae are oxidase (-).
Other than MacCaonkey, one can use Eosin-Methylene Blue to arrive at
the same conclusions.
I let somone with expertise handle the antibiotic testing part!!
Comments and corrections welcome!
Hope the cat gets better!
David J. McGee
MCGEED at hal.hahnemann.edu
Graduate Student
McPHU (Medical College of Pennsylvania and Hahnemann University)
Center City Campus
15th and Vine Streets
Dept. Microbiology and Immunology M.S. 410
New College Building RM 10302
Philadelphia, PA 19102
Phone: 215-762-8275
Fax: 215-762-1004
Happy 1995!
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