We are doing some work with different isolates of Pseudomonads, some of
which we know what they are and some we don't. I would like to put in
place some simple diagnostic system to verify which strains we are
working on. I don't really care that I find out precisely what they
are, but I want to be able to unambiguously distinguish between a few
strains on a repeatable basis. In other words we are doing some
genetics and transferring plasmids between strains and I want to be
sure that my putative strain from a cross is really a new exconjugant
and not a contaminant or revertant. There are no useful phenotypes in
hand (except on the plasmid).
Are there some diagnostic strips or microtiter dish systems that I can
utilize that have lots of different tests so that I most likely can
distinguish between these strains.
Suggestions or pointers would be quite welcome.
Michael Benedik benedik at uh.edu
Biochemical Sciences
University of Houston
Houston, TX 77204-5934