In the referenced article, rogers at usuhsb.usuhs.mil writes:
>In Article <36ptvg$gd at mserv1.dl.ac.uk>
>"RICHARD A. MURPHY 312-996-8630" <U14663 at UICVM.UIC.EDU> writes:
>>When reading ODs of bacterial cultures with a spectrophotometer, what
>>wavelength should be used and is it the same for all bacteria?.
>>Dick Murphy
>>+-------------------------------------------------------------------+
>>|Richard A. Murphy Bitnet: U14663 at uicvm.bitnet |
>>|University of Illinois at Chicago Internet: U14663 at uicvm.uic.edu |
>>>+-------------------------------------------------------------------+
>We use 550nm on E. coli and 660nm on L. pneumophila
>
Just out of interest, what is the basis for the various wavelengths used to
measure bacterial culture density and what are the pros and cons of each. For
example, lots ODs are measured at 595nm others at 600nm. Why?
Gordon Munro
Bath University
UK
