IUBio

Can you pick up a bacterium?

Robert Morrell Jr. bmorrell at ISNET.IS.WFU.EDU
Mon Oct 24 08:35:08 EST 1994


On 24 Oct 1994, BCapstone wrote:
> contaminated by urethral flora.  After seeing this on gram stain I think
> that if Enterobacter Cloacae is really present as the pathogen, then the
> cultures are being overgrown by Staph as the cocci are so numerous in
> comparison.  I would like to separate the rods out and culture them to see
> if I can give the patient a better chance.

I have tried to get this point across before, but again:

E. cloacae is not a difficult organism to grow. Rather than laser 
manipulation, try a 30 cent MacConkey agar plate. No matter how much 
staph is there, the Enterobacter will grow out pure and observable. In 
truth, even a moderately observent microbiologist can see a single colony 
of enteric like E cloacae on a plate =covered= in staph.  The only way 
micromanipulation is useful is if the rod you are seeing is not the E 
cloacae but some other organism with similar growth requirements as the 
staph and a much slower grower. In this case the connection between the E 
cloacae sepsis and the prostatitis is bogus. Also since you have 
recovered the cloacae, you already know its antibiogram, and can treat 
accordingly.

So I ask more pointed: exactly what is the purpose of this exercise?

Bob Morrell




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