In article <g-yu1.738.2EA2A7E2 at uxa.cso.uiuc.edu>, g-yu1 at uxa.cso.uiuc.edu (guoyun yu) writes:
> Hello,
> I got a spontaneous mutant of Bacillus sp. which requires
> streptomycin to growth in NA medium. I call it streptomycin-dependent mutant.
> Are there any reports on this strange phenome? Please give me information.
Hi there,
I think the first reports about streptomycin resistance and dependence
appeared in the 1960's (look out for reviews by Luigi Gorini).
In E. coli streptomycin dependence is caused by certain mutations in the
gene rpsL (which codes for ribosomal protein S12). These mutations often
lead to an increased accuracy and decreased efficiency of translation.
A strong decrease in efficiency can result in a reduction of the maximal
bacterial growth rate. (Does your mutant grow slower?) There is an
extensive literature available about E. coli rpsL mutants that cause
streptomycin resistance or dependence (Advice: in the older literature
they are called strA mutations...). I know that in other bacteria, as
Mycobacteria tuberculosis, streptomycin resistance is also caused by
alterations of S12, and I guess that the same holds true for Bacillus.
If you want some literature references conntact me by:
redaschi at ubaclu.unibas.ch
