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I need some help with a sequencing problem.
I want to obtain a vector for sequencing a PCR
product which is preferably high copy number
and does not have ampicillin or tetracycline
resistance as a marker.
it is for use in E. coli .
it should have standard cloning sites, specifically
EcoRI.
I have tried the ususal source sand contacted
pretty much every comopany but no luck.
if anybody can help then please post a message
or email me at f.r.byrne at abdn.ac.uk
Any help gratefully received.
thanks.
Fergus Byrne
Aberdeen University, Scotland.
