In article <mpowell-111094084233 at levinmac3.nichd.nih.gov> mpowell at lmgvax.nichd.nih.gov (Mike Powell) writes:
>From: mpowell at lmgvax.nichd.nih.gov (Mike Powell)
>Subject: Re: soil bacteria
>Date: Tue, 11 Oct 1994 13:42:33 GMT
>In article <1704BE896S85.MIKEFER at UNIVSCVM.CSD.SCAROLINA.EDU>,
>MIKEFER at UNIVSCVM.CSD.SCAROLINA.EDU wrote:
>>>> As a portion of a micro lab, I have had my students isolate bacteria from
>> soil. We are now in the process of identifying the isolates. Pseudomonas
>> and Bacillus spp. are predominant, but we have isolated a small Gram - cocci
>> (tiny). Can anyone provide a list of typically isolated bacteria from soil?
>> Any suggestions as to the identity of the Gram negative cocci?
>> Our isolation method has selected for aerobes and facultative anaerobes that
>> are non-fastidious (grow well on trypic soy agar at 37 C).Thanks in advance.
Dear Mike
Gram-negative cocci from soil are sought of unusual as most Gram neg. bugs
tend to be rods. Check the gram-stain to see if they are consistently
coccus-shaped. If they are truly cocci the strain could belong to the genus
Paracoccus which is fairly common in soil. Paracoccus strains are non-motile,
strictly aerobic, oxidase positive and colonies have a white to an off
white pigmentation. Paracoccus strains can denitrify (nitrate is reduced to
nitrogen gas) a very distinctive trait of the genus. Other possibilities exist
but you may have to consult Bergey's Manual for additional information. Any
other questions please send e-mail jbowman at utkvx.utk.edu,
Good Luck !
John Bowman Ph D.
Univ. Tennessee
>
