I am a Ph.D looking for a postdoc position in a laboratory studying
molecular pathogenesis and/or antibiotic resistence in bacteria. I have
10 years experience with molecular biology and strong backgrounds in
microbiology/biochemistry. The following is my CV.
Zhengyu Sha
ADDRESS
Home Office
1494 Hawthorn Court 2188 Molecular Biology Building
Ames, Iowa 50010 Department of Zoology and Genetics
Phone:(515) 296-8376 Iowa State University, Ames, IA 50011
Fax:(515) 294-0345 Phone:(515) 294-4210
Email address: zsha at iastate.edu
STATUS Permanent Resident
CAREER A Postdoctoral Position in a Research
OBJECTIVE Institute/University
EDUCATION Doctor of Philosophy: Sept., 1994
Iowa State University Ames, Iowa
Major: Molecular Biology, GPA: 3.3/4.0
Thesis Title: "Purification and cloning of a periplasmic catalase from
B. abortus and the oxidative stress in the pathogenesis of Brucella"
Master of Science: Sept. 1986
Major: Genetic Engineering GPA: 3.6/4.0
Bachelor of Science: Sept. 1983
Major: Biology GPA: 3.4/4.0
Fudan University Shanghai, PRC
EMPLOYMENT Research and Teaching Assistant 1989 to present
Dept. of Zoology and Genetics, Iowa State University, Ames,Iowa
Project: Purified a catalase from B.abortus, cloned the gene
and studied the biological significance of oxidative
stress in the pathogenesis of Brucella
Techniques: -Protein purification, conventional chromatography,
HPLC, protein sequencing, immunological and
enzyme assays, protein analysis
-DNA library screening, subcloning, PCR, DNA
sequencing, heterologous gene expression ,
site-directed mutagenesis
-transposon, mapping and other bacterial genetics
Teaching: Lectured a genetic engineering lab course and a
biology lab course
Lab Supervisor 1987-1989
Reijing Hospital, Shanghai, PRC
Project: Involved in a preclinical trial of a recombinant hGH
and developed the procedures to diagnose several
genetic diseases
Techniques: Southern blotting, RFLP, ELISA
Researcher Associate 1986-1987
Biotechnology Center, Shanghai, PRC
Project: Improved a recombinant Hepatitis B vaccine
Techniques: Eukaryotic expression system
Teaching and Research Assistant 1983-1986
Genetic Institute, Fudan University, Shanghai, PRC
Project: Studied the polymorphism of D.melanogaster
Techniques: -Isozyme electrophoresis
-RFLP of Mitochondria DNA
Teaching: Lectured a genetic lab course
PUBLICATION Zhengyu Sha, Thomas. J. Stabel, John E. Mayfield(1994).
Cloning of a periplasmic catalase from Brucella abortus.
Journal of Bacteriology. (in press)
Thomas. J. Stabel, Zhengyu Sha, John Mayfield(1994).
Periplasmic location of Brucella abortus Cu/Zn superoxide
dismutase. Vet. Microbiol.38: 307-314
Zhengyu Sha, Fred M. Tatum, John E. Mayfield(1994).Analysis of
the survival of a catalase-deletion mutant of Brucella
abortus in vitro and in vivo. ( in preparation)
AWARDS PREMIUM FOR ACADEMIC EXCELLENCE (PACE Award)
Iowa State University 1989-1990
PROFESSIONAL American Society for Microbiology
MEMBERSHIP
REFERENCES Dr. John E Mayfield
Professor, Department of Zoology & Genetics
Iowa State University
Ames, Iowa 50010
Tel: (515)-294-6847
Dr. Duane M. Enger
Chairman, Department of Zoology & Genetics
Iowa State University
Ames, Iowa 50010
Tel: (515)-294-0320
Thomas J. Stabel
Staff Scientist, Physiopathology Unit
National Animal Disease Center, ARS, USDA
P.O. Box 70
Ames, Iowa 50010
Tel: (515)- 239-8292
Research Interests Statement
Research Interests
My current research focuses on the stress response and protein
localization in the Gram-negative bacterium, B.abortus . In the future,
I wish to continue to pursue a challenging career in the development of
new technology applicable to the biotechnology industry that will
ultimately benefit the clinical treatment and prevention of human
disease.
Research Experience
I started to work on the molecular mechanisms of B.abortus
pathogenesis in my Ph.D. project in 1990. My initial goal was to test
the hypothesis that the antioxidant enzymes such as catalase play an
important role in the survival and the propagation of facultative
intracellular bacteria. Mammalian phagocytic cells release reactive
oxidants such as superoxide anion and hydrogen peroxide when they
confront invading bacteria The oxidants are believed to be involved in
antibacterial activity of phagocytic cells by placing an oxidative
stress on the bacteria. The facultative intracellular bacteria such as
B. abortus must respond to and protect themselves form this oxidative
stress to survive in the host. I thought it was very likely that
Brucella cells would be found to have a periplasmic catalase which could
detoxify the hydrogen peroxide generated by host phagocytic cells. First
I developed a new method for isolation of the periplasmic proteins from
Brucella cells while retaining the biologic activity. Then I
successfully isolated a periplasmic catalase from B. abortus , purified
it to homogeneity and investigated its biochemical characteristics.
Using an antibody against the purified enzyme, I screened a B. abortus
DNA library, cloned this catalase gene, and sequenced the gene. Most
recently I constructed a catalase null mutant of B. abortus by
site-directed mutagenesis and characterized its phenotype. The mutant
strain is sensitive to hydrogen peroxide stress and was retarded growth
when inoculated into Balb/c mice. My data indicate that the periplasmic
catalase is important for B. abortus to survive under the hydrogen
peroxide stress.
In my Master degree project in China, I used isozyme
electrophoresis and RFLP of mitochondria DNA to study the polymorphism
of D.melanogaster subspecies in eastern China. After graduating with a
M.S. degree, I worked in a project to develop a vaccine against
Hepatitis B virus using vaccinia virus as a live carrier expression
system. Later, I developed PCR-based methods for diagnosing genetic
disorders such as human growth hormone(hGH) deficiency. During lab
rotations in ISU, I was involved in studying the AC/DS transposon in
soybean, and I used a transposon to introduce a gene marker into
Staphylococcus aureus chromosome for chromosome mapping.
