Thanks. I should have mentioned that I'm aware of the Tn10 derivatives in
the Methods in Enzymology article and I have talked to both Kleckner's
technician and Bob Simons, but neither group has any Sm or Sp resistant
mini-Tn10s. I know that a Sm/Sp resistant mini-Tn10 was constructed
several years ago by a group in Europe, but I can't seem to find anyone
who has it. We could construct one from Tn10s and plasmids we have in our
freezer, but I thought that would be a waste of time and effort if we
could get it in the mail. --> Stanley
In article <1994Nov14.081307.1 at jaguar.csc.wsu.edu>,
f0380903 at jaguar.csc.wsu.edu wrote:
> In article <s-maloy-1111941704260001 at maloy3.life.uiuc.edu>, s-maloy at uiuc.edu> (Stanley Maloy) writes:
> > Does anyone know where we could get a Tn10 derivative with Sp or Sm
> > resistance (or any other selectable marker besides Tc, Km, Cm, Ap, or
> > Hg)?
> >
> > We want to do transposon mutagenesis in a Salmonella typhimurium strain
> > with a Tn10 held chromosomal duplication and a Km resistant Mud fusion
> > with a linked Tn10dCm insertion (for backcrosses) and saving Ap
> > sensitivity (because we may want to bring in a Ap resistant plasmid for in
> > vivo cloning of any interesting chromosomal mutants).
> >
> > Thanks.
> >
> > --
> > Stanley Maloy
> > Department of Microbiology
> > University of Illinois
> > 131 Burrill Hall, 407 S. Goodwin Ave
> > Urbana, IL 61801
> In Methods in Enzymology v. 204 Kleckner et al have an article that describes
> Tn10 derived mini-Tn's. They have an element that contains a Kan gene
that can
> be used to generate Kan-translational fusions. It also has a selectable
> erythromycin marker on it, it is available on an Ap-r pBR322 derivative.
>> Matthew L. Nilles (F0380903 at jaguar.csc.wsu.edu)
> Dept. of Microbiology
> Washington State University
> Pullman, WA 99164
--
Stanley Maloy
Department of Microbiology
University of Illinois
131 Burrill Hall, 407 S. Goodwin Ave
Urbana, IL 61801
